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Gastropoda, with approximately 80,000 extent species, is the largest class of Mollusca. Among gastropods, apple snails (Ampullariidae) are ecologically and economically important, with approximately 120 species in nine genera that are distributed in tropical and subtropical freshwater ecosystems world-wide. Members of this family exhibit diverse morphological and physiological adaptations to their living environments, which make them ideal candidates for studying population divergence, speciation and biogeography. Some species of apple snails native to South America have been introduced into Asia, North America and some Pacific islands, causing tremendously damage to semi-aquatic agriculture. Their success in invasion has been partly attributed to their adaptation to extreme environmental conditions. To facilitate molecular studies using apple snails, we sequenced the transcriptome of seven species of apple snails. Together with the transcriptome of another recently sequenced species, our data cover representatives from five genera. They included New World (i.e., Asolene platae, Marisa cornuarietis, Pomacea diffusa, P. scalaris, P. canaliculata and P. maculata) and Old World (i.e., Lanistes nyassanus and Pila ampullacea) species that diverged roughly 100 million years ago as Gondwana split due to continental drift. Total RNA was extracted from multiple tissue types separately, and sequenced in paired-end mode on an Illumina HiSeq sequencer. De novo transcriptome was conducted for the eight species of apple snails, resulting in 20,730 to 35,828 unigenes for each species, with N50 value ranging from 1,320 to 1,803 bp. On average, 75.9% of these unigenes were functionally annotated by NCBI, GO, and KEGG. AmpuBase, a relational database, was constructed for sharing the transcriptome data. User-friendly features of AmpuBase include BLAST search with DNA or protein sequence, keyword search for unigene or functional term, and downloadable selected sequences and whole transcriptome. More details of the research background, methods and results can be found in Ip et al. AmpuBase: A transcriptome database for eight species of apple snails (Gastropoda: Ampullariidae) (submitted). |
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| Dr. Jian-Wen Qiu | Dr. Xin Huang |
| Department of Biology | Department of Computer Science |
| Hong Kong Baptist University | Hong Kong Baptist University |
Email: qiujw hkbu.edu.hk | Email: xinhuanghkbu.edu.hk |